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Blood Serum (/ˈsɪərəm/) is the fluid and solute component of blood which does not play a role in clotting.^[1] It may be defined as blood plasma without fibrinogens. Serum includes all proteins not used in blood clotting; all electrolytes, antibodies, antigens, hormones; and any exogenous substances (e.g., drugs or microorganisms). Serum does not contain white blood cells (leukocytes), red blood cells (erythrocytes), platelets, or clotting factors.

The study of serum is serology. Serum is used in numerous diagnostic tests as well as blood typing. Measuring the concentration of various molecules can be useful for many applications, such as determining the therapeutic index of a drug candidate in a clinical trial.^[2]

To obtain serum, a blood sample is allowed to clot (coagulation). The sample is then centrifuged to remove the clot and blood cells, and the resulting liquid supernatant is serum.^[3]

Serum is an essential factor for the self-renewal of embryonic stem cells in combination with the cytokine leukemia inhibitory factor.^{[citation needed]}

Clinical and laboratory uses[edit]

The serum of convalescent patients successfully recovering (or already recovered) from an infectious disease can be used as a biopharmaceutical in the treatment of other people with that disease, because the antibodies generated by the successful recovery are potent fighters of the pathogen. Such convalescent serum (antiserum) is a form of immunotherapy.

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Serum is also used in protein electrophoresis, due to the lack of fibrinogen which can cause false results.

Purification strategies[edit]

Blood serum and plasma are some of the largest sources of biomarkers, whether for diagnostics or therapeutics. Its vast dynamic range, further complicated by the presence of lipids, salts, and post-translational modifications, as well multiple mechanisms of degradation, presents challenges in analytical reproducibility, sensitivity, resolution, and potential efficacy. For analysis of biomarkers in blood serum samples, it is possible to do a pre-separation by free-flow electrophoresis that usually consists of a depletion of serum albumin protein.^[4] This method enables greater penetration of the proteome via separation of a wide variety of charged or chargeable analytes, ranging from small molecules to cells.

Usage note[edit]

Like many other mass nouns, the word serum can be pluralized when used in certain senses. To speak of multiple serum specimens from multiple people (each with a unique population of antibodies), physicians sometimes speak of sera (the Latin plural, as opposed to serums).

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See also[edit]

References[edit]

• Martin, Elizabeth A., ed. (2007). Concise Medical Dictionary (7th ed.). Oxford, England: Oxford University Press. ISBN978-0-19-280697-0. Retrieved 8 September 2009.
• Wang, Wendy; Srivastava, Sudhir (2002). 'Serological Markers'. In Lester Breslow (ed.). Encyclopedia of Public Health. 4. New York, New York: Macmillan Reference USA. pp. 1088–1090.

1. ^'serum'. The Free Dictionary. Retrieved 2019-10-06.
2. ^Kaplan, Larry (2005-10-06). 'Serum Toxicology'(PDF). Clinical Pathology/Laboratory Medicine 2005. Columbia University. Retrieved 2020-01-28.
3. ^Thavasu PW, Longhurst S, Joel SP, Slevin ML, Balkwill FR (1992). 'Measuring cytokine levels in blood. Importance of anticoagulants, processing, and storage conditions'. J Immunol Methods. 153 (1–2): 115–24. doi:10.1016/0022-1759(92)90313-i. PMID1381403.CS1 maint: multiple names: authors list (link)
4. ^Nissum M., Foucher AL.: Analysis of human plasma proteins: a focus on sample collection and separation using free-flow electrophoresis. In: Expert Rev Proteomics. Nr. 5, 2008, S. 571–87.

External links[edit]

This article describes some of the commonly used features of Activity Monitor, a kind of task manager that allows you see how apps and other processes are affecting your CPU, memory, energy, disk, and network usage.

Open Activity Monitor from the Utilities folder of your Applications folder, or use Spotlight to find it.

Overview

The processes shown in Activity Monitor can be user apps, system apps used by macOS, or invisible background processes. Use the five category tabs at the top of the Activity Monitor window to see how processes are affecting your Mac in each category.

Add or remove columns in each of these panes by choosing View > Columns from the menu bar. The View menu also allows you to choose which processes are shown in each pane:

• All Processes
• All Processes Hierarchically: Processes that belong to other processes, so you can see the parent/child relationship between them.
• My Processes: Processes owned by your macOS user account.
• System Processes: Processes owned by macOS.
• Other User Processes: Processes that aren’t owned by the root user or current user.
• Active Processes: Running processes that aren’t sleeping.
• Inactive Processes: Running processes that are sleeping.
• Windowed Processes: Processes that can create a window. These are usually apps.
• Selected Processes: Processes that you selected in the Activity Monitor window.
• Applications in the last 8 hours: Apps that were running processes in the last 8 hours.

CPU

The CPU pane shows how processes are affecting CPU (processor) activity:

Click the top of the “% CPU” column to sort by the percentage of CPU capability used by each process. This information and the information in the Energy pane can help identify processes that are affecting Mac performance, battery runtime, temperature, and fan activity.

More information is available at the bottom of the CPU pane:

• System: The percentage of CPU capability currently used by system processes, which are processes that belong to macOS.
• User: The percentage of CPU capability currently used by apps that you opened, or by the processes those apps opened.
• Idle: The percentage of CPU capability not being used.
• CPU Load: The percentage of CPU capability currently used by all System and User processes. The graph moves from right to left and updates at the intervals set in View > Update Frequency. The color blue shows the percentage of total CPU capability currently used by user processes. The color red shows the percentage of total CPU capability currently used by system processes.
• Threads: The total number of threads used by all processes combined.
• Processes: The total number of processes currently running.

You can also see CPU or GPU usage in a separate window or in the Dock:

• To open a window showing current processor activity, choose Window > CPU Usage. To show a graph of this information in your Dock, choose View > Dock Icon > Show CPU Usage.
• To open a window showing recent processor activity, choose Window > CPU History. To show a graph of this information in your Dock, choose View > Dock Icon > Show CPU History.
• To open a window showing recent graphics processor (GPU) activity, choose Window > GPU History. Energy usage related to such activity is incorporated into the energy-impact measurements in the Energy tab of Activity Monitor.

Memory

The Memory pane shows information about how memory is being used:

More information is available at the bottom of the Memory pane:

• Memory Pressure: The Memory Pressure graph helps illustrate the availability of memory resources. The graph moves from right to left and updates at the intervals set in View > Update Frequency. The current state of memory resources is indicated by the color at the right side of the graph:
  • Green: Memory resources are available.
  • Yellow: Memory resources are still available but are being tasked by memory-management processes, such as compression.
  • Red: Memory resources are depleted, and macOS is using your startup drive for memory. To make more RAM available, you can quit one or more apps or install more RAM. This is the most important indicator that your Mac may need more RAM.
• Physical Memory: The amount of RAM installed in your Mac.
• Memory Used: The total amount of memory currently used by all apps and macOS processes.
  • App Memory: The total amount of memory currently used by apps and their processes.
  • Wired Memory: Memory that can’t be compressed or paged out to your startup drive, so it must stay in RAM. The wired memory used by a process can’t be borrowed by other processes. The amount of wired memory used by an app is determined by the app's programmer.
  • Compressed: The amount of memory in RAM that is compressed to make more RAM memory available to other processes. Look in the Compressed Mem column to see the amount of memory compressed for each process.
• Swap Used: The space used on your startup drive by macOS memory management. It's normal to see some activity here. As long as memory pressure is not in the red state, macOS has memory resources available.
• Cached Files: Memory that was recently used by apps and is now available for use by other apps. For example, if you've been using Mail and then quit Mail, the RAM that Mail was using becomes part of the memory used by cached files, which then becomes available to other apps. If you open Mail again before its cached-files memory is used (overwritten) by another app, Mail opens more quickly because that memory is quickly converted back to app memory without having to load its contents from your startup drive.

For more information about memory management, refer to the Apple Developer website.

Energy

The Energy pane shows overall energy use and the energy used by each app:

• Energy Impact: A relative measure of the current energy consumption of the app. Lower numbers are better. A triangle to the left of an app's name means that the app consists of multiple processes. Click the triangle to see details about each process.
• Avg Energy Impact: The average energy impact for the past 8 hours or since the Mac started up, whichever is shorter. Average energy impact is also shown for apps that were running during that time, but have since been quit. The names of those apps are dimmed.
• App Nap: Apps that support App Nap consume very little energy when they are open but not being used. For example, an app might nap when it's hidden behind other windows, or when it's open in a space that you aren't currently viewing.
• Preventing Sleep: Indicates whether the app is preventing your Mac from going to sleep.

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More information is available at the bottom of the Energy pane:

• Energy Impact: A relative measure of the total energy used by all apps. The graph moves from right to left and updates at the intervals set in View > Update Frequency.
• Graphics Card: The type of graphics card currently used. Higher–performance cards use more energy. Macs that support automatic graphics switching save power by using integrated graphics. They switch to a higher-performance graphics chip only when an app needs it. 'Integrated' means the Mac is currently using integrated graphics. 'High Perf.' means the Mac is currently using high-performance graphics. To identify apps that are using high-performance graphics, look for apps that show 'Yes' in the Requires High Perf GPU column.
• Remaining Charge: The percentage of charge remaining on the battery of a portable Mac.
• Time Until Full: The amount of time your portable Mac must be plugged into an AC power outlet to become fully charged.
• Time on AC: The time elapsed since your portable Mac was plugged into an AC power outlet.
• Time Remaining: The estimated amount of battery time remaining on your portable Mac.
• Time on Battery: The time elapsed since your portable Mac was unplugged from AC power.
• Battery (Last 12 hours): The battery charge level of your portable Mac over the last 12 hours. The color green shows times when the Mac was getting power from a power adapter.

As energy use increases, the length of time that a Mac can operate on battery power decreases. If the battery life of your portable Mac is shorter than usual, you can use the Avg Energy Impact column to find apps that have been using the most energy recently. Quit those apps if you don't need them, or contact the developer of the app if you notice that the app's energy use remains high even when the app doesn't appear to be doing anything.

Disk

The Disk pane shows the amount of data that each process has read from your disk and written to your disk. It also shows 'reads in' and 'writes out' (IO), which is the number of times that your Mac accesses the disk to read and write data.

The information at the bottom of the Disk pane shows total disk activity across all processes. The graph moves from right to left and updates at the intervals set in View > Update Frequency. The graph also includes a pop-up menu to switch between showing IO or data as a unit of measurement. The color blue shows either the number of reads per second or the amount of data read per second. The color red shows either the number of writes out per second or the amount of data written per second.

To show a graph of disk activity in your Dock, choose View > Dock Icon > Show Disk Activity.

Network

The Network pane shows how much data your Mac is sending or receiving over your network. Use this information to identify which processes are sending or receiving the most data.

The information at the bottom of the Network pane shows total network activity across all apps. The graph moves from right to left and updates at the intervals set in View > Update Frequency. The graph also includes a pop-up menu to switch between showing packets or data as a unit of measurement. The color blue shows either the number of packets received per second or the amount of data received per second. The color red shows either the number of packets sent per second or the amount of data sent per second.

To show a graph of network usage in your Dock, choose View > Dock Icon > Show Network Usage.

Cache

In macOS High Sierra 10.13.4 or later, Activity Monitor shows the Cache pane when Content Caching is enabled in the Sharing pane of System Preferences. The Cache pane shows how much cached content that local networked devices have uploaded, downloaded, or dropped over time.

Auto-tuned singers real voices live. The husband of the couple, Mike, had the software on his home computer – he dabbles in music production – and the idea was that we’d record a song together, then Auto-Tune it.We looked for something with four-part harmony, so we could all sing, and for a song where the backing instrumental was available online. We settled on Boyz II Men’s “End of the Road.” One by one we went into the bedroom to record our parts, with a mix of shame and titillation not unlike taking turns with a prostitute.When we were finished, Mike played back the finished piece, without Auto-Tune. It was nerve wracking to listen to, I felt like my entire body was cringing.

Use the Maximum Cache Pressure information to learn whether to adjust Content Caching settings to provide more disk space to the cache. Lower cache pressure is better. Learn more about cache activity.

The graph at the bottom shows total caching activity over time. Choose from the pop-up menu above the graph to change the interval: last hour, 24 hours, 7 days, or 30 days.

Learn more

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• Learn about kernel task and why Activity Monitor might show that it's using a large percentage of your CPU.
• For more information about Activity Monitor, open Activity Monitor and choose Help > Activity Monitor. You can also see a short description of many items in the Activity Monitor window by hovering the mouse pointer over the item.